Gingiguard

Advanced Pet Oral Care

GingiGuard Gel is an innovative veterinary oral care product designed to simplify pet dental health.

Utilizing cutting-edge biofilm-targeting technology, GingiGuard Gel effectively protects your pet’s teeth and gums with just one easy application per month.

Say goodbye to daily brushing routines and hello to healthier, happier pets.

Revolutionizing Dental Care in Dogs

After a rigorous five-year investigation, the Topikos Biofilm Research Institute (TBRI) has unveiled a significant leap in treating periodontitis, based on extensive research with over 100 dogs suffering from gingivitis and periodontitis. This pioneering work has laid the foundation for a novel treatment approach that promises to transform dental health management in dogs, marking a pivotal moment in veterinary dental science.

How It Works

GingiGuard Gel works by selectively inhibiting the growth of harmful bacteria that contribute to biofilm formation. These biofilms are the root cause of many dental problems in pets. By disrupting this process, GingiGuard Gel helps to prevent conditions like gingivitis, periodontal disease, and bad breath, all while being gentle and safe for pets.

Research

Under the guidance of our experts, Dr. Kevin Cawood, Dr. William Cast, and Dr. Michael Bechill, the trial employed a dual approach of traditional dental care coupled with our groundbreaking antibiofilm compound. This trial aims to illuminate the path towards a healthier future for our canine companions, assessing the compound’s efficacy in fostering significant oral health improvements.

A one-year-old Belgian Malinois was diagnosed with advanced periodontitis, featuring a chronic lesion in the furcation area. Forty-five days post-administration, the treatment solution exhibited prolonged adherence to tissue, lasting approximately a month. This sustained engagement underscores the solution’s lasting impact.

Microbial Growth Inhibition, Including Staphylococcus aureus

The MBEC assay (Minimum Biofilm Eradication Concentration) is a laboratory method used to assess how effective antimicrobial treatments are against bacteria that form protective biofilms, like Staphylococcus aureus.

Our active molecule, TKS-203, can fully inhibit the growth of the S. aureus biofilm at a concentration of 0.25 percent, highlighting its potential as an effective solution for combating stubborn biofilm-related infections.

We subsequently carried out a 142-dog field study of the clinical effectiveness of GingiGuard against controls with supporting scientific data.

Canine Study Overview

Our research was designed as a field study encompassing 142 animals of diverse breeds, ages, sexes, and sizes. This methodology not only enhances the ecological validity of the findings but also allows for an understanding of canine health and behavior in real-world settings.

Demographics

The study population included dogs of various sizes and breeds, enhancing the relevance of findings across a wide range of canine profiles.

Microbial Shifts in Oral Cavity

The Krona graphs depict the taxonomic classification of bacteria found in the oral cavity pre- and post-treatment, illustrating substantial shifts in microbial populations after a single application of GingiGuard. These changes correlate with clinical improvements, such as reduced gingivitis, gum bleeding, and bad breath. Photos of the oral cavity further demonstrate these outcomes.

Pre-Treatment

Post-Treatment

Operational Taxonomic Unit (OTU) Analysis:

Definition: OTUs represent unique types of organisms, classified by their genetic similarities. This is essential for understanding microbial communities.

Effect of Dental Cleaning Alone: Dental cleaning alone did not significantly alter the number or diversity of OTUs, indicating minimal impact on the oral microbial community.

Impact of GingiGuard Treatment: Administration of GingiGuard resulted in significant changes in OTU diversity and composition, highlighting its direct effect on bacterial populations.

Impact on Microbial Species

Changes in the abundance of specific microbial species provide valuable insights into oral health outcomes in dogs.
A star (*) indicates significant changes in microbial populations between baseline and four weeks after treatment.
Colors represent comparative changes in relative abundance of specific microbes.

Prevention of Gingivitis Return

Black line (●): Dogs that only received dental cleaning experienced a quicker return of bad breath, with 40-50% affected by 6 months.

Blue line (■): Dogs that received dental cleaning plus GingiGuard showed a much slower return, with fewer than 10% affected even by 10 months.

This highlights that GingiGuard reduces the return of bad breath compared to dental cleaning alone.

Prevention of Bad Breath Return

Black line (●): In dogs that only received dental cleaning, about 25-30% showed a return of gingivitis after 6 months.

Blue line (■): For dogs that received dental cleaning plus GingiGuard, fewer than 10% showed signs of gingivitis even by 10 months.

This demonstrates that GingiGuard significantly delays and reduces gingivitis recurrence compared to dental cleaning alone.

Safety Evaluation

A safety study was independently conducted by Attentive Science, LLC to evaluate GingiGuard’s tolerance in dogs. Following the initial treatment, efficacy was evaluated over several months using next-generation sequencing (NGS), offering a comprehensive understanding of the treatment’s long-term effects.

Study Participants: 4 dogs, including two males and two females. Comprehensive physical examinations were conducted pre-dosing and at 1, 24, and 72 hours following administration.
Dosing Method: Gingival administration, once daily for a single day across three successive escalating doses, with a 7-day washout period between doses.
Observational Findings: No clinical signs related to the treatment were observed.
Irritation Assessment: No changes in irritation scores, including erythema, edema, or ulceration, were associated with the treatment.
Maximum Dose Safety: A top dose of 5 mg/mL of the GingiGuard active ingredient was locally administered to canine gingiva with no adverse effects.
Maximum Dose Safety: A top dose of 5 mg/mL of the GingiGuard active ingredient was locally administered to canine gingiva with no adverse effects

Promising Results

The study showcased remarkable findings: a single application of the treatment solution notably cleared periodontal disease symptoms within approximately two weeks. Serial next-generation sequencing (NGS) studies confirmed the sustained health of treated dogs over six months, highlighting improved symptoms and breath. Moreover, the treatment’s efficacy was not limited by the dog’s size, breed, or initial disease severity, indicating its broad applicability. These results signal a significant advancement in treating periodontitis and gingivitis, offering a new beacon of hope for pet health care.

Key Benefits

Long-Lasting Protection: With GingiGuard Gel, a single application lasts up to a month, providing continuous protection against harmful biofilms and plaque buildup.
Scientifically Proven Formula: GingiGuard Gel disrupts the biofilms that cause dental diseases, targeting the root cause of plaque formation and keeping your pet’s mouth healthy.
Easy to Apply: The gel is designed for effortless application directly to your pet’s gums, making it stress-free for both you and your pet.

Implications for Veterinary Medicine

The TBRI study’s outcomes could significantly alter veterinary dental practices by introducing an effective, non-invasive treatment for periodontal diseases. This could lead to wider adoption of preventive care routines and minimize the need for surgeries, thereby enhancing overall pet health and potentially extending the life expectancy of animals suffering from dental issues. The implications of this research stretch beyond immediate dental health, suggesting a holistic improvement in the well-being of pets due to reduced pain and infection risk.